(A) UM1 cells were treated with miR-7 mimic for 0, 24 or 48 h. The levels of total Akt [Akt(pan)], p-Akt (at Thr308 and Ser473) and p-ERK1/2 were examined by Western blotting as described in the Experimental section. (B) UM1 cells were stimulated with IGF1 for 0, 3, 10 and 30 min. The levels of total Akt and p-Akt (at Thr308 and Ser473) were examined by Western blotting using specific antibodies. (C) Cells were pre-treated with the PI3K inhibitor LY294002 (50 μM for 1 h), miR-7 mimic (100 nM for 48 h) or control mimic (100 nM for 48 h) and then stimulated with IGF1 for 0, 3, 10 and 30 min. The levels of p-Akt (at Thr308 and Ser473) were examined by Western blotting. ACTB, β-actin.