Table 1. Predicted miR-7 targets involved in Akt and EGFR signalling.
Targets for miR-7 were predicted using Target Scan Human 5.1. PCT, probability of conserved targeting.
| Gene symbol | Gene name | Conserved sites
|
Poorly conserved sites
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total | 8-mer | 7-mer-m8 | 7-mer-1A | Total | 8-mer | 7-mer-m8 | 7-mer-1A | Aggregate PCT | Total context score | References | ||
| IGF1R | Insulin-like growth factor 1 receptor | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 2 | 0.63 | −0.17 | |
| IRS1 | Insulin receptor substrate 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0.59 | −0.52 | [10] |
| IRS2 | Insulin receptor substrate 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0.94 | −0.60 | [10] |
| PIK3CD | Phosphoinositide 3-kinase catalytic subunit δ | 1 | 1 | 0 | 0 | 3 | 1 | 1 | 1 | 0.79 | −0.69 | |
| EGFR | Epidermal growth factor receptor | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0.57 | −0.78 | [10,11] |
| RAF1 | v-raf-1 murine leukaemia viral oncogene homologue 1 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0.80 | −0.68 | [11] |
| PAK1* | p21/CDC42/RAC1-activated kinase 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0.14 | −0.29 | [12] |
*
No conserved miR-7-targeting site was predicted by TargetScan in PAK1 mRNA sequence. A poorly conserved miR-7-target site was predicted in the 3′-UTR of the PAK1 gene. The miR-7 interaction with this target site was experimentally validated previously [12].