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Viral dose effect on transudation: hHSCs transduced with lentivirus at cell to virus ratio of 1:1000, 1:2000; 1:5000. Highest level of GFP expression was found in 1:5000 cell to virus ratio and also higher level of cell death was found at this dose. 1:2000 ratio was the optimal dose for good level of transgene expression as well as low level of cell death (see Figure 3).
