Figure 1. Redirecting T cell specificity by insertion of genes encoding a TCR or CAR.

Peripheral blood T cells (or selected subsets) are activated and exposed to a gene delivery vector to introduce a tumor targeting T cell receptor (TCR) or chimeric antigen receptor (CAR). In the case of TCR introduction, the possibility of mispairing with endogenous α and β chains leads to the potential for four receptors to be expressed on the transduced cell.