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. Author manuscript; available in PMC: 2011 Jul 6.
Published in final edited form as: Methods Mol Biol. 2010;666:87–102. doi: 10.1007/978-1-60761-820-1_7

Fig. 7.2.

Fig. 7.2

Growth of biofilm on saliva-coated hydroxyapatite (sHA) disks. A Schematic showing how to make the disk holder using orthodontic wire, B hydroxyapatite disks and custom-made holder, C sHA disks placed in a vertical position inside the wells of a microtiter plate, D growth of biofilms using LMWS medium, e typical 5-day-old biofilm formed on sHA disk.