Table 1.
Sequences of primers used in the Real-time RT-PCR
| Name | GenBank accession no. | Sequnce(5′-3′) | |
|---|---|---|---|
| β-actin | NM_007393.3 | Forward Reverse |
GGAGATTACTGCCCTGGCTCCTA GACTCATCGTACTCCTGCTTGCTG |
| aggrecan | NM_007424.2 | Forward Reverse |
CGCCACTTTCATGACCGAGA TCATTCAGACCGATCCACTGGTAG |
| osteocalcin | NM_001032298.2 | Forward Reverse |
CTTGAAGACCGCCTACAAAC GCTGCTGTGACATCCATAC |
| osterix | NM_130458.3 | Forward Reverse |
CGCATCTGAAAG CCCACTTG CAGCTCGTCAG AGCGAGTGAA |
| TRAP | NM_007388.3 | Forward Reverse |
TTGCGACCATTGTTAGCCACATA TCAGATCCATAGTGAAA CCGCAAG |
| MMP9 | NM_013599.2 | Forward Reverse |
CCATGCACTGGGCTTAGATCA GGCCTTGGGTCAGGCTTAGA |
| RANK | NM_009399.3 | Forward Reverse |
ATGGTGGGCTACCCAGGTGA ACTTGCGGCTGCACAGTGA |
| cathepsin K | NM_007802.3 | Forward Reverse |
CAGCAGAACGGAGGCATTGA CTTTGCCGTGGCGTTATACATACA |
| MMP13 | NM_008607.2 | Forward Reverse |
ACTTTGTTGCCAATTCCAGG TTTGAGAACACGGGGAAGAC |
| Col 10 | NM_009925.4 | Forward Reverse |
ACCCCAAGGACCTAAAGGAA CCCCAGGATACCCTGTTTTT |
| ALP | NM_007431.2 | Forward Reverse |
ACACCTTGACTGTGGTTACTGCTGA CCTTGTAGCCAGGCCCGTTA |
Note: TRAP: Tartrate-resistant acid phosphatase; ALP: Alkaline phosphatase; MMP9: Matrix metallopeptidase 9; MMP13: Matrix metallopeptidase 13; RANK: Receptor activator of nuclear factor kappa-B; Col 10: Type X collagen.