Table 5.
Sex Chromosome Genes Assayed with TaqMan Real-Time PCR in Dorsolateral Prefrontal Cortex
| Gender | Gene copy mean (SD)
|
|||
|---|---|---|---|---|
| RPS4Ya,b | SMCYb | DBYb | XISTb | |
| Female | 33 (28) | −506 (320) | 5.9 (8.0) | 34 640 (8297) |
| Male | 53 200 (21 370) | 9813 (3208) | 4002 (820) | 281 (97) |
| t-testc | 0.005 | 0.002 | 0.002 | 0.0007 |
The mean gene copy number (SD) are shown for RPS4Y, SMCY, DBY, and XIST in male and female samples.
a
The RPS4Y clone was also used as a reference standard in the assay for copy number, and values for male 36 000 (15 427) and female 30.8 (19.3) with the same results as genomic DNA standard as shown in the table. The RT− sample yielded a negligible copy number of 0.2 and 0.8 for male and female mean.
b
Genomic DNA standard with RT− sample copy number subtracted from RT+ copy number. A negative value indicates that genomic presence in total RNA sample gives a reading higher than the RT+ sample.
c
t-Test p-values unequal variance between groups.