Figure 1.

Stimulus-dependent dynamics of p53 in response to NCS (DSBs) and to UV. (A–D) Representative traces of MCF7 cells expressing p53-Venus exposed to 100 (A) or 400 ng/ml (C) of NCS or to 2 (B) or 8 J/m² (D) UV at the indicated time following treatment. (E, F) p53-Venus levels averaged over the traces of cells in response to various levels of NCS and UV. Note that for NCS, the averaged response is shown only for the first p53 pulse, as synchrony between cells is lost in subsequent pulses. Each experiment includes between 60 and 110 cells. The mean trace was normalized to the mean p53-Venus of the first time point. (G, H) Quantification of the relative amplitude and full-width half-maximum duration of all p53 pulses in response to NCS (G) or the first pulse in response to UV (H). Error bars represent s.e.m.