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. Author manuscript; available in PMC: 2011 Jul 6.
Published in final edited form as: Gastroenterology. 2007 May 21;133(2):619–631. doi: 10.1053/j.gastro.2007.05.018

Figure 10.

Figure 10

(A) Effects of G-CSF on OC proliferation. OCs were incubated in IMDM medium supplemented with 10% fetal bovine serum (□), or serum-free IMDM medium containing 0.5% bovine serum albumin with G-CSF at 10 ng/mL (◆), 100 ng/mL (■), 500 ng/mL (▲), or without G-CSF (○, negative control) for the indicated times. Differences were statistically significant when comparing 100 ng/mL G-CSF vs negative controls. *P < 0.05, **P < .005. (B) Effect of G-CSF on OC migration in transwells. OCs were seeded in the top chamber with 10, 100, or 500 ng/mL G-CSF placed in the bottom chamber, or 100 ng/mL of G-CSF placed in both chambers (CNT+/+, chemokinetic control). Migration controls were used with no G-CSF in either chamber (cnt−/−). Data represent the mean value + SD of 3 independent experiments, normalized with respect to control migration (relative chemotactic index; *P < .05, **P < .01, ***P < .0001). When G-CSF was added to the lower chamber, OCs crossed the filter in a dose-dependent manner, reaching a peak in the presence of 100 ng/mL of G-CSF (>6-fold increase after 6 hours).