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(A–C) Confocal microscopy of double-immunofluorescence staining of Thy-1+ sorted cell cytospins for (A) G-CSFR (green), (B) OV6 (red), and (C) co-expression (yellow). Note that the distribution of these proteins within the cell is not identical (large and small arrows, respectively). Cell nuclei were stained with DAPI (blue). Confocal magnification, 252× (63× objective combined with 4× digital zoom).
