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. 2001 Apr 15;29(8):1724–1732. doi: 10.1093/nar/29.8.1724

Figure 1.

Figure 1

A single point mutation in the conserved ATP-binding motif of Rhp51 impairs its DNA repair activity. (A) Comparison of amino acid sequences of conserved ATP-binding motif A from E.coli RecA, S.cerevisiae Rad51, human Rad51 and S.pombe Rhp51. (B and C) rhp51+ and rhp51 K155A carried by multicopy plasmids were subjected to survival tests to determine their complementation of MMS (B) and UV (C) sensitivity of rhp51Δ cells. Diamond, wild-type cells with vector Splac551; square, rhp51Δ with Splac551; triangle, rhp51Δ with p51.551; circle, rhp51Δ with p51.551 K155A.