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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2011 Jul;70(7):568–577. doi: 10.1097/NEN.0b013e31821ff8d8

Figure 5.

Figure 5

Knockdown of YAP1 decreases cell growth in glioblastoma cell lines. (A-C) YAP1 mRNA expression was knocked down in glioblastoma cell lines U87 (A), U373 (B), and GBM1 (C) using the lentiviral vector pLKO.1 expressing a shYAP1. The decrease in YAP1 mRNA was monitored by Q-PCR (left). Cellular growth was evaluated using the MTS assay (right). The growth relative to starting value was graphed for each cell line transduced with either empty pLKO.1 vector (solid line) or pLKO.1-shYAP1 (hatched lines). Statistical analysis was performed using the Student's t test at each time point. (D-F) The ability of YAP1 knockdown to modulate the hedgehog targets PTCH1B (D), GLI1 (E), and GLI2 (F) in glioblastoma cell lines GBM1, U373, and U87 was assessed by Q-PCR. Statistical analysis was performed using the Student t-test. Asterisks (*) in all graphs represent significance below the p = 0.05 level. Error bars represent the standard error of the mean for 3 to 6 replicates.