. Author manuscript; available in PMC: 2012 Aug 1.

Published in final edited form as: Protein Expr Purif. 2011 Apr 24;78(2):139–142. doi: 10.1016/j.pep.2011.04.011

Table 1.

Proteases used in this study.

Protease	Cleavage Site
Enterokinase	Asp-Asp-Asp-Lys^▾
Factor Xa	Ile-Glu/Asp-Gly-Arg^▾
HRV3C	Leu-Glu-Val-Leu-Phe-Gln^▾ Gly-Pro
SUMOstar	Recognizes tertiary structure of SUMOstar tag (10kDa)
TEV	Glu-Asn-Leu-Tyr-Phe-Gln^▾ Gly/Ser
Thrombin	Leu-Val-Pro-Arg^▾ Gly-Ser

The amino acid recognition site for each protease is provided with the site of cleavage indicated by the

^▾

All protease recognize short, linear sequences while SUMOstar recognizes the tertiary structure of the SUMOstar tag.