Table 2.
Summary of detergent sensitivity of proteases.
| Name | [Det] mM | Abbrev. | Enterokinase | Factor Xa | HRV 3C | SUMOstar | TEV | Thrombin |
|---|---|---|---|---|---|---|---|---|
| ZWITTERGENT®3–12 | 8.4 (2.8) | Z3–12 | ||||||
| ZWITTERGENT®3–14 | 10 (0.2) | Z3–14 | ||||||
| n-Decyl-N,N-dimethylglycine | 38 (19) | DMG | ||||||
| n-Dodecyl-N,N-dimethylglycine | 4.5(1.5) | DOMG | ||||||
| n-Decyl-N,N-dimethylamine-N-oxide | 21 (10.5) | DAO | ||||||
| n-Undecyl-N,N,-dimethylamine-N-oxide | 9.6 (3.2) | UDAO | ||||||
| n-Dodecyl-N,N-dimethylamine-N-oxide | 3 (1) | LDAO | ||||||
| C-DODECAFOS™ | 44 (22) | C-DDFOS | ||||||
| CYCL0F0S™-4 | 28 (14) | CF-4 | ||||||
| CYCL0F0S™-5 | 13.5 (4.5) | CF-5 | ||||||
| CYCL0F0S™-6 | 8.04 (2.68) | CF-6 | ||||||
| CYCL0F0S™-7 | 6.2 (0.62) | CF-7 | ||||||
| FOS-CHOLINE®-10 | 22 (11) | FC-10 | ||||||
| F0S-CH0LINE®-11 | 5.55 (1.85) | FC-11 | ||||||
| F0S-CH0LINE®-12 | 4.5 (1.5) | FC-12 | ||||||
| F0S-CH0LINE®-13 | 7.5 (0.75) | FC-13 | ||||||
| F0S-CH0LINE®-14 | 6 (0.12) | FC-14 | ||||||
| F0S-CH0LINE®-IS0-11 | 53.2 (26.6) | FC-I11 | ||||||
| F0S-CH0LINE®-IS0-11-6U | 51.6 (25.8) | FC-I11-6U | ||||||
| F0S-CH0LINE®-IS0-9 | 64 (32) | FC-I9 | ||||||
| FOS-CHOLINE®-UNSAT-11-10 | 15.5 (6.2) | FC-U10-11 | ||||||
| 1,2-Diheptanoyl-sn-glycero-3-phosphocholine | 4.2 (1.4) | DHPC | ||||||
| LysoPC-10 | 20 (8) | LPC-10 | ||||||
| LysoPC-12 | 7 (0.7) | LPC-12 | ||||||
| F0SFEN™-9 | 4.05 (1.35) | FOSFEN-9 | ||||||
| CHAPS | 20 (8) | CHAPS | ||||||
| CHAPSO | 20 (8) | CHAPSO | ||||||
| n-Dodecvl-N,N-(dimethylammonio)undecanoate | 6.5 (0.13) | DDMAU | ||||||
| n-Dodecyl-N,N-(dimethylammonio)butyrate | 12.9 (4.3) | DDMAB | ||||||
| LAPAO | 4.8 (1.6) | LAPAO | ||||||
| TRIPAO | 13.5 (4.5) | TRIPAO | ||||||
| TWEEN®20 | 5.9 (0.059) | T-20 | ||||||
| BRIJ®35 | 9.1 (0.091) | BRIJ-35 | ||||||
| TRITON®X-10 | 11.5 (0.23) | TX-10 | ||||||
| TRITON®X-114 | 10 (0.2) | TX-114 | ||||||
| TRITON®X-305 | 6.5 (0.65) | TX-305 | ||||||
| TRITON®X-405 | 8.1 (0.81) | TX-405 | ||||||
| [Octylphenoxy] polyethoxyethanol | 15 (0.3) | NID-P40 | ||||||
| Dimethyloctylphosphine oxide | 80 (40) | AP08 | ||||||
| Dimethylnonylphosphine oxide | 20 (10) | AP09 | ||||||
| Dimethyldecylphosphine oxide | 14.0 (4.7) | APO10 | ||||||
| Dimethylundecylphosphine oxide | 3.6 (1.2) | AP011 | ||||||
| Dimethyldodecylphosphine oxide | 5.7 (0.57) | AP012 | ||||||
| Triethylene glycol monohexyl ether | 46 (23) | C6E3 | ||||||
| Tetraethylene glycol monohexyl ether | 60 (30) | C6E4 | ||||||
| Pentaethylene glycol monohexyl ether | 74 (37) | C6E5 | ||||||
| Pentaethylene glycol monoheptyl ether | 42 (21) | C7E5 | ||||||
| Tetraethylene glycol monooctyl ether | 20 (8) | C8E4 | ||||||
| Pentaethylene glycol monooctyl ether | 17.75 (7.1) | C8E5 | ||||||
| Hexaethylene glycol monooctyl ether | 25 (10) | C8E6 | ||||||
| Pentaethylene glycol monodecyl ether | 8.1 (0.81) | C10E5 | ||||||
| Hexaethylene glycol monodecyl ether | 9 (0.9) | C10E6 | ||||||
| Polyoxyethylene(9)decyl ether | 3.9 (1.3) | C10E9 | ||||||
| Octaethylene glycol monododecyl ether | 9 (0.09) | C12E8 | ||||||
| Polyoxyethylene(9)dodecyl ether | 5 (0.05) | C12E9 | ||||||
| Polyoxyethylene(10)dodecyl ether | 10 (0.2) | C12E10 | ||||||
| Polyoxyethylene(8)tridecyl ether | 10 (0.1) | C13E8 | ||||||
| Big CHAP | 8.7 (2.9) | CHAP | ||||||
| Big CHAP, deoxy | 4.2 (1.4) | CHAP-D | ||||||
| Octyl-2-hydroxyethyl-sulfoxide | 48.4 (24.2) | OHES | ||||||
| Rac-2,3-dihydroxypropyloctylsulfoxide | 48.4 (24.2) | RDHPOS | ||||||
| Genapol®X-100 | 7.5 (0.15) | GX-100 | ||||||
| n-Heptyl-β-D-thioglucopyranoside | 58 (29) | HTG | ||||||
| n-Octyl-β-D-glucopyranoside | 36 (18) | OG | ||||||
| n-Nonyl-β-D-glucopyranoside | 16.25 (6.5) | NG | ||||||
| CYGLU®-3 | 56 (28) | CYGLU-3 | ||||||
| HECAMEG | 39 (19.5) | HECAMEG | ||||||
| Hega®-9 | 78 (39) | HEGA-9 | ||||||
| C-Hega®-10 | 70 (35) | C-HEGA-10 | ||||||
| C-Hega®-11 | 23 (11.5) | C-HEGA-11 | ||||||
| CYMAL®-3 | 60 (30) | CYMAL-3 | ||||||
| CYMAL®-4 | 19 (7.6) | CYMAL-4 | ||||||
| CYMAL®-5 | 7.2 (2.4) | CYMAL-5 | ||||||
| CYMAL®-6 | 5.6 (0.56) | CYMAL-6 | ||||||
| CYMAL®-7 | 9.5 (0.19) | CYMAL-7 | ||||||
| 2,6-Dimethyl-4-heptyl-β-D-maltoside | 55 (27.5) | DMHM | ||||||
| n-Octyl-β-D-maltopyranoside | 39 (19.5) | OM | ||||||
| n-Nonyl-β-D-maltopyranoside | 15 (6) | NM | ||||||
| n-Decyl-α-D-maltopyranoside | 4.8 (1.6) | DαM | ||||||
| n-Decyl-β-D-maltopyranoside | 5.4 (1.8) | DM | ||||||
| n-Undecyl-α-D-maltopyranoside | 5.8 (0.58) | UDαM | ||||||
| n-Undecyl-β-D-maltopyranoside | 5.9 (0.59) | UDM | ||||||
| ω-Undecylenyl-β-D-maltopyranoside | 3.6 (1.2) | ωUDM | ||||||
| n-Dodecyl-α-D-maltopyranoside | 7.5 (0.15) | DDαM | ||||||
| n-Dodecyl-β-D-maltopyranoside | 8.5 (0.17) | DDM | ||||||
| n-Tridecyl-β-D-maltopyranoside | 1.5 (0.03) | TDM | ||||||
| n-Octyl-β-D-thiomaltopyranoside | 21.25 (8.5) | OTM | ||||||
| n-Nonyl-β-D-thiomaltopyranoside | 9.6 (3.2) | NTM | ||||||
| n-Decyl-β-D-thiomaltopyranoside | 9 (0.9) | DTM | ||||||
| n-Undecyl-β-D-thiomaltopyranoside | 10.5 (0.21) | UDTM | ||||||
| n-Dodecyl-β-D-thiomaltopyranoside | 5 (0.05) | DDTM | ||||||
| Sucrose8 | 48.8 (24.4) | S-8 | ||||||
| Sucrose10 | 7.5 (2.5) | S-10 | ||||||
| Sucrose12 | 15 (0.3) | S-12 |
The membrane protein detergent panel is shown above. The values in parenthesis in the [Det] column are the CMC values for each detergent. Detergents in bold were purchased from Avanti Polar Lipids, italics from Bachem, underlined from EMD Biosciences, and all others from Anatrace. The legend shows the relative protease activity in each detergent based on the amount of cleavage product observed on the protein gel.