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. Author manuscript; available in PMC: 2011 Jul 6.
Published in final edited form as: Vaccine. 2011 Feb 22;29(17):3299–3309. doi: 10.1016/j.vaccine.2011.02.019

Figure 1.

Figure 1

Demonstration of influenza-specific CD4+ T cell cross-reactivity against the S-OIV. CD4+ T cells were isolated from frozen PBMCs and cultured with purified autologous DCs for 24 hrs. Where indicated, the DCs were left untouched or pre-pulsed overnight with vaccine or protein. Peptides, individually or together as a pool, were added directly to the DC/T cell coculture well. Thereafter, antigen-specific T cells were detected by IFNγ ELISPOT assay. Eight donors were included in this evaluation.