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. Author manuscript; available in PMC: 2011 Jul 6.
Published in final edited form as: Vaccine. 2011 Feb 22;29(17):3299–3309. doi: 10.1016/j.vaccine.2011.02.019

Figure 2.

Figure 2

Demonstration of robust cross-reactive influenza-specific CD4+ T cells effector responses against predicted S-OIV HA epitope sequences. DCs were pulsed with no antigen or Brisbane HA overnight and then co-cultured with purified autologous CD4+ T cells. After 12 days, the cultures were harvested and restimulated with fresh autologous DCs that had, in some cases, been pre-pulsed overnight with Brisbane HA. Some assay wells were pulsed directly with S-OIV HA peptides or the negative control TT peptide, TT947–967. After 7 hr, the lymphocytes were evaluated by flow cytometry using a conventional intracellular staining protocol. The dot plots show live/CD4-gated lymphocytes.