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Evaluation of the purity of the cell membrane isolation method. Cells were subjected to crude isolation of Membrane (M) and Cytosol (C) fractions. Western blot analysis of the different markers - (A) Na/K-ATPase (plasma membrane), (B) GRP94 (endoplasmic reticulum), (C) Ku86 (nuclear) and (D) GAPDH (cytosol). (M) Membrane, (C) Cytosol and (Mw) molecular weight marker (kDa).
