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. 2011 May 30;9:73. doi: 10.1186/1477-7827-9-73

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Copyright ©2011 Yap et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Representative 2D SDS-PAGE image of proteins from the human endometrial carcinoma epithelial cell line (ECC-1). Proteins (150 μg) extracted from membrane fraction of ECC-1 cells were separated by 2D PAGE (first dimension pH 3-10, second dimension 8-16% polyacrylamide gradient). (A) A representative gel of the pooled internal standard (Cy2) is shown. Overall 866 spots were resolved. Proteins were clustered according to similarities in their expression patterns (grouped by colours). (B) Differentially expressed proteins were identified and spots of interest were chosen for further analysis by LCMS/MS are numbered and indicated by circles.