Figure 1.

Autocaller plot showing the fluorescence of 22 samples (green and dark blue) and two nontemplate controls (light blue) in the M203 assay. The TaqMan probe complementary to the ancestral allele (G) was labelled with the VIC fluorophore, whereas FAM was used to label the derived allele (C). Three clusters are seen in the plot: the samples with the derived allele, thus belonging to the Y-chromosome haplogroup branch DE, are shown in dark blue; the samples with the ancestral allele, thus belonging to any haplogroup except DE, are shown in green; and the negative controls are shown in light blue.