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. 2011 Jun 10;6:71. doi: 10.1186/1748-717X-6-71

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Copyright ©2011 Firat et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Correlation of proliferation with γIR-induced mitotic catastrophe and late apoptosis in SLGCs. A. Analyses of CD133+ and CD133- immature glioma cells. Flow cytometry analysis of CD133 expression in magnetic bead-separated CD133+/CD133-populations of GBM8 SLGCs (upper left). Expression of CD133, Sox2, musashi and nestin as determined by Western blotting (upper right). Viable and dead cells (lower left) and cells with signs of mitotic catastrophe (lower right) 7 d after irradiation. B. Influence of EGF plus FGF-2 on proliferation, mitotic catastrophe, and apoptosis of GBM4 SLGCs. The cells were incubated with or without EGF plus FGF-2 (20 ng/ml). After 24 h the cells were irradiated, and 7 d later analyzed. In the cytokine-treated cultures, EGF and FGF-2 were replenished after 3 days. Mean ± S.D. of at least three experiments is shown; statistical significance (p < .05).