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. 2011 Jun 7;6:40. doi: 10.1186/1750-1326-6-40

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Copyright ©2011 Taravini et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Astrocytes over-express pleiotrophin after AdPTN infection. (A) Coronal plates from the Paxinos and Watson atlas illustrating the site of injection of the adenoviruses (left) and the region of the substantia nigra from which the microphotographs have been taken (right). (B) Immunohistochemical detection of PTN on coronal sections at mesencephalic level after AdPTN or Adβgal injection, 7 and 14 days post-injection, in adult normal rats. The strong PTN-immunoreactivity in glial-like cells is only seen in animals treated with AdPTN. The squares in the low magnification images indicate the area shown in the high magnification ones (bottom panel of images). Scale bars: 500 μm (low magnification images) and 100 μm (high magnification images). (C) Double immunohistochemistry showing the co-localization of GFAP and PTN in astrocytes in a control rat 7 days after AdPTN injection. Scale bar: 50 μm.