Table 1. qRT–PCR primers.
| Gene | Direction | Primer sequence |
|---|---|---|
|
K12 |
Forward |
CCAGGTGAGGTCAGCGTAGAA |
|
|
Reverse |
CCTCCAGGTTGCTGATGAGC |
|
K13 |
Forward |
CTGAACAAGGAGGTGTCTACCA |
|
|
Reverse |
ATAGCGGCACTCCGTCTCT |
|
K15 |
Forward |
ACCACCACATTTCTGCAAACT |
|
|
Reverse |
AGCTGAGATACTTCGGCTTCC |
|
K19 |
Forward |
TGAGTGACATGCGAAGCCAAT |
|
|
Reverse |
ACCTCCCGGTTCAATTCTTCA |
|
Mucin 5AC |
Forward |
CAGCCACGTCCCCTTCAATA |
|
|
Reverse |
ACCGCATTTGGGCATCC |
|
GAPDH |
Forward |
GGCTGAGAACGGGAAGCTTGTCAT |
| Reverse | CAGCCTTCTCCATGGTGGTGAAGA |
Abbreviations: Cytokeratin, K; Glyceraldehyde 3-phosphate dehydrogenase, GAPDH.