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. 2011 Jul 6;6(7):e20605. doi: 10.1371/journal.pone.0020605

Figure 4. Glioma regions dominated by recruited cells can become independent of PDGFR signaling.

Figure 4

(a–d) DAPI-stained frozen section of an Ntv-a RCAS-PSG-induced GBM heterozygous deleted for Ink4a/Arf and Pten at tumor initiation, containing large areas of recruitment expressing IGFR (b, red) and EGFR (c, white); (d) shows composite. (e) Quantification of growth factor receptor expression in Ntv-a GBMs in homozygous (n = 22) or heterozygous deleted backgrounds, containing large regions of progeny cells (“progeny”, n = 43) or recruited cells (“recruited” n = 32), by immunostaining. (f) Percent of Ki67-positive cells in untreated or PTK787-treated Ntv-a GBMs heterozygous deleted at glioma initiation and containing large regions of progeny (green squares) or recruited (blue squares) cells. Recruited cells in RCAS-PSG-induced Ntv-a GBMs heterozygous deleted for Ink4a, Arf, Pten at tumor initiation and containing large recruited areas do not reduce their proliferation rates in response to treatment with PDGFR/VEGFR inhibitor PTK787, suggesting that they become independent of PDGFR signaling.