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. 2011 Jul 6;6(7):e21880. doi: 10.1371/journal.pone.0021880

Figure 3. LDLR deletion increases Aβ deposition in the mouse hippocampus.

Figure 3

A. Immunohistochemistry for total Aβ (6E10) in the brains of female mice. Representative pictures are shown for each genotype. The Aβ deposition is increased in the subiculum of the 5XFAD/LDLR-/- mice compared to the 5XFAD mice. The same effect is observed in the 5XFAD/ApoE-/-LDLR-/- mice where Aβ deposition is more intense in the subiculum. Aβ deposition in the 5XFAD/ApoE-/-LDLR-/- cortices appears more ‘dense’ and “compact” compared to the 5XFAD/ApoE-/- mice where Aβ deposition is more “diffuse”. Scale bar 0.5 mm (n = 5−7, 6–7 sections per animal, 240 mm apart). B. Quantitation of Aβ immunoreactivity in the hippocampi (left) of the analysed groups shows a significant increase in the Aβ deposition in the 5XFAD/LDLR-/- and the 5XFAD/ApoE-/-LDLR-/- mice. In the cortices (right) there is no significant difference between 5XFAD and 5XFAD/LDLR-/- as well as between 5XFAD/ApoE-/- and 5XFAD/ApoE-/-/LDLR-/-.*P<0.05. P-values among all groups are analysed in Table 2 and Table 3 for hippocampi and cortices respectively. C. Quantitation of Aβ42 and Aβ40 levels by ELISA in the 5XFAD and the 5XFAD/LDLR-/- mouse brain extracts showed an increase both in the guanidine and lysis fraction in the 5XFAD/LDLR-/- mice. A similar increase was also observed in the guanidine fraction of the 5XFAD/ApoE-/-LDLR-/- mice compared to the 5XFAD/ApoE-/- but not in the lysis fraction (n = 5−7) *P<0.05, **P<0.001.