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. 2011 Jul 6;6(7):e21994. doi: 10.1371/journal.pone.0021994

Figure 4. Stably transfected SK-N-SH cells consist of glial cells only.

Figure 4

(A) Morphology of untransfected SK-N-SH cells or (B) stably transfected SK-N-SH cells. The G418 (Roche) antibiotic selection procedure for selection of positive STOX1A and MOCK stable transfected cells resulted by clonal selection in a homogeneous cell population that is composed of the glial (S-type) type. (C) Immunophenotype of untransfected SK-N-SH cells showing intense membrane staining for GD2 but lack nuclear calcylin staining (E). No GD2 membrane staining was observed in stably transfected SK-N-SH cells (D) while cells show high calcyclin nuclear staining (F). Green: GD2, Red: Calcyclin, Blue: DAPI.