Table 1. Gene locus, protein name, gene bank reference sequence, features, gene conservation, sequence of the primers employed for DNA amplification, and molecular mass of expressed recombinant proteins.
| Gene locus1 | Recombinant protein given name | NCBI reference sequence number2 | Description/Function | Conservation (identity)3 | Sequence of primers for PCR amplification | Recombinant protein molecular mass |
| LIC10258 | rLIC10258 | YP_000249 | Hypothetical protein with ompA domain | Lai (99%); LBH (79%) | F:5′ GGATCC GAAGCCTTCTCACCCAATTG 3′ (BamH I) | 65.74 kDa |
| R:5′ CCATGGTTAAAGTGAAAGATAAAAATCGATTC 3′ (Nco I) | ||||||
| LIC12880 | Lp30 | YP_002796 | Putative lipoprotein | Lai (99%); LBH (76%) | F: 5′ CTCGAG GAAGTTGTCCGAGTCTAT 3′ (Xho I) | 30.68 kDa |
| R: 5′ CCATGG TTATTGATTGTTTAATTCAG 3′ (Nco I) | ||||||
| LIC12238 | rLIC12238 | YP_002173 | Hypothetical protein | Lai (99%); LBH (77%); | F: 5′ CTCGAG TGTTTTAAACCTACCGGAG 3′ (Xho I) | 17.635 kDa |
| LBP (39%) | R:5′AAGCTTCTACTTCATCGCTTTTTCTATATC 3′ (Hind III) |
3
http://www.ncbi.nlm.nih.gov/blast/Blast.cgi/. This work, Lai: L. interrogans serovar Lai; LBH: L. borgpetersenii serovar Hardjo-bovis; LBP: L. biflexa serovar Patoc.