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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Dev Biol. 2011 May 10;356(1):40–50. doi: 10.1016/j.ydbio.2011.05.002

Fig. 5.

Fig. 5

Lack of rugae specific-morphogen expression in β-Catenin cKO. (A–F) Shh in situ at stages indicated. (G, H) Ptch1 in situ on E13.5 control and β-Catenin cKO sections. (I–N) Bmp7 in situ at stages indicated. (O, P) SEM on E14.5 control and Shh cKO palates (P). β-Catenin cKO palate at the same stage was presented as an inset in (P) for comparison. (Q) In WT palate, active Wnt signaling (red) in rugae establishes a Shh gradient (blue), which guides palatal growth along the A–P axis (quad arrow). As palate elongates and the distance between R1 and Rn increases, ruga Rn+1 emerges in-between them (dashed line). In β-Catenin cKO, absence of Wnt and subsequent Shh morphogen expression results in unregulated tissue expansion, leading to undergrowth anteroposteriorly and overgrowth mediolaterally. Scale bars: A–F and I–N: 0.5mm; in G and H: 0.25mm; in O and P: 1mm.