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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: Toxicon. 2011 Feb 24;59(4):472–486. doi: 10.1016/j.toxicon.2011.02.020

Table 1. Disintegrin-integrin binding kinetics by fluorescence polarization.

The binding kinetics were calculated by measuring the steady state binding of FITC-labeled disintegrins to either purified (αvβ3 and αvβ5) or recombinant (α5β1) functional human integrins. The dissociation constants for interactions of either CN or VCN with soluble integrins were determined by Scatchard analysis using a non-linear curve fit.

Disintegrin Integrin Kd (+/−SD)
αvβ3 α5β1 αvβ5
CN 6.6nM (0.8) 191.3nM(65.2) 19.5nM (5.7)
VCN 7.4nM (0.4) 15.2nM (4.2) 41.2nM (12.3)