Table 1.
Useful reagents to specifically label the cellular (SPG, PG, fat body) and acellular (neural lamella) layers of Drosophila BBB.
Physiologic Markers |
Antibody | GAL4 / UAS-GFP | Genetrap | |
---|---|---|---|---|
Neural Lamella | Lectin | -- | -- | Trol (G00022) |
Fat Body | >100kDa dextran (histiocytes) Nile red (triglycerides) | -- | Croquemort-GAL4 (FBst0025041) | -- |
Perineurial Glia | <100kDa dextran | VMAT B5 | UAS-moesin-GFP1 | -- |
UAS-tau-GFP2 | ||||
UAS-actin-GFP1 | ||||
Subperineurial Glia | -- | C219 (Mdr65)3 | SPG-GAL44 | Nrg (G00305) |
C494 (unknown)5,6 | Nrx-IV (CA06597) | |||
Moody7 | Dlg1 (CC01936) |
(Bainton, unpublished),