Table 2.
Of the 550 P-GAL4 lines tested in our live retinal imaging screen, the 31 genes listed here showed reproducible retinal phenotypes. The eight barrier-specific genes are grouped by the layers in which they are specifically expressed (as revealed by our follow-up anatomical assays). BLAST homology was determined by inverse PCR, and instances where the P-element inserted in between two loci are noted by a slash. Driver number = Heberlein Lab, personal communication. Fold Δ = fluorescent dextran uptake relative to WT controls.
| Name | Symbol | CG no. | Driver | Fold Δ | Notes | Reference |
|---|---|---|---|---|---|---|
| Fat Body Layer | ||||||
| sarah | sra | CG6072 | 11–165 | 1.4 | Inhibitor of calcineurin | (Chang et al. 2003) |
| Perineurial Glial Layer | ||||||
| I'm not dead yet | Indy | CG3979 | 11–67 | 1.3 | Citrate transmembrane transporter | (Inoue et al. 2002; Knauf et al. 2002) |
| Ranbp 21 / CG14215 | Ranbp 21 / CG14215 | CG12234 / CG14215 | 8–79 | 1.7 | Intracellular protein transport | IPR001494 |
| varicose / CG9328 | vari / CG9328 | CG9326 / CG9328 | 10–12 | 1.8 | Septate junction assembly | (Wu et al. 2007) |
| CG6424 | CG6424 | CG6424 | 10–72 | 1.2 | -- | -- |
| Subperineurial Glial Layer | ||||||
| Gliotactin / CG3793 | Gli / CG3793 | CG3903 / CG3793 | 4–7 | 1.6 | Septate junction assembly | (Auld et al. 1995; Schulte et al. 2003) |
| Neu3 | Neu3 | CG7649 | 2–35 | 1.2 | Metalloendopeptidase activity | IPR001590 |
| snRNA U2 at 38ABb | snRNA:U2:38ABb | CR32878 | 10–37 | 1.9 | Nuclear mRNA splicing | -- |
| Non-Barrier Specific | ||||||
| alpha-Man-IIb | alpha-Man-IIb | CG4606 | 8–176 | 2.2 | Carbohydrate binding, Alpha-mannosidase | IPR011013, UniProt P27046 |
| cactus | cact | CG5848 | 7–67 | 1.0 | I-kappaB homolog | (Geisler et al. 1992) |
| dorsal | dl | CG6667 | 7–11 | 0.9 | NF-kappaB homolog | (Ghosh et al. 1990) |
| Glycerol-3-phosphate dehydrogenase | Gpdh | CG9042 | 9–167 | 1.1 | -- | -- |
| Kruppel homolog 2 | Kr-h2 | CG9159 | 7–61 | 2.0 | Integral to membrane | IPR005344 |
| PNGase-like | Pngl | CG7865 | 2–15 | 1.5 | Carbohydrate binding | (Funakoshi et al. 2010) |
| RNA pol. II 215kD subunit | RpII215 | CG1554 | 6–26 | 1.0 | DNA-directed RNA polymerase | -- |
| CG10778 | CG10778 | CG10778 | 5–55b | 1.1 | Phagocytosis, engulfment | (Stroschein-Stevenson et al. 2006) |
| CG11131 / CG11133 | CG11131 / CG11133 | CG11131 / CG11133 | 4–28 | 1.1 | CG11133 is ATP-dep helicase | IPR011545 |
| CG13624 | CG13624 | CG13624 | 7–63 | 1.6 | Protein homodimerization, Transcription factor | (Fassler et al. 2002), IPR004827 |
| CG1753 | CG1753 | CG1753 | 7–35 | 0.9 | Cysteine biosynthesis | IPR001216 |
| CG32666 | CG32666 | CG32666 | 4–76b | 1.0 | Cell adhesion, Reg’n of cell shape | (Kiger et al. 2003) |
| CG4068 | CG4068 | CG4068 | 4–54a | 1.2 | -- | -- |
| CG5953 | CG5953 | CG5953 | 2–76 | 1.0 | -- | -- |
| CG6785 | CG6785 | CG6785 | 10–69 | 1.0 | -- | -- |
| CG8399 | CG8399 | CG8399 | 5–34a | 0.8 | Dopamine beta-monooxygenase | IPR013050 |
| HMS-Beagle()116 | -- | -- | 5–61 | 1.4 | Transposable element | -- |
| No gene detected | -- | -- | 2–60, 4–10, 5–128, 6–65, 8–194, 9–203 | |||