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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Dev Biol. 2011 May 11;356(1):19–27. doi: 10.1016/j.ydbio.2011.05.004

Figure 2.

Figure 2

Effect of 6OS-depleted heparin on embryonic kidney branching morphogenesis. A: Graphical analysis of the average number of UB tips as a percentage of control when cultured in the presence of varying concentrations of heparin or 6OS-depleted heparin. Mean ± SD, n>3, *p<0.05 compared to control. B-D: Phase contrast photomicrographs of whole embryonic kidneys cultured for 4-6 days in the absence (B) or presence of 100 μg/ml of either de6OS-heparin (C) or heparin (D). Even at higher concentrations, compared to heparin-treated kidneys (C), UB architecture is largely preserved despite further reduction in UB tip number in the presence of de6OS (B; inset is a confocal image of the embryonic kidney; open arrow points to UB tip and closed arrow points to UB stalk (D. biflorus lectin (green) and E-cadherin (red)). Photomicrographs taken at 10X magnification.