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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Dev Biol. 2011 May 11;356(1):19–27. doi: 10.1016/j.ydbio.2011.05.004

Figure 5.

Figure 5

Known branching morphogens have stronger binding affinity to 6-O sulfate moieties over 2-O sulfate moieties. Western blots of the various fractions from the columns demonstrate that the stimulatory activity corresponds to presence of pleiotrophin (A) and heregulin (B), known potent branching morphogens (Sakurai et al., 2001; Sakurai et al., 2005). When purified growth factors, FGF1 (C) and GDNF (D) are run over the columns, similar to PTN and HRG, there is a progressive downward shift in binding affinity from the heparin column to the 2OS-depleted heparin column to the de6OS column, demonstrating that 6-O sulfate residues are required for high affinity binding.