Fig. 3.
Effect of the bacterial extract (BE) on the tyrosinase activity and the tyrosinase and MITF expressions. (A) The Melan-a cells were incubated with 10 mM L-DOPA for 90 min and the absorbance was measured at 475 nm. The values indicate the mean of five independent experiments±SD. *p<0.01. The tyrosinase (B) and MITF (C) protein expressions of the Melan-a cells treated with bacterial extract (50µg/ml) were determined by western blotting.