Fig. 1.
CD22 deficiency causes hyperproliferation of B cells in response to TLR ligands. a CFSE-labeled B cells were cultured for 3 days in the absence (black) or presence (gray) of 5.0 μg/ml poly(I:C), 1.5 μg/ml LPS, 15 n M CpG and 3.0 μg/ml anti-IgM. Cells were analyzed by flow cytometry. b Percentage of dividing cells from 2 days' culture subtracted with the value in the absence of stimulation are shown. Statistical analyses were performed by Student's t test. * p < 0.05; * * p < 0.01; * * * p < 0.001. c Living cell number in the wells of 3 days' culture is shown. Before the analysis, 1.0 × 104 of beads was added to the culture well. Cells were then harvested and analyzed by flow cytometry. Living cell number was calculated by the formula: living cell number in the well = the number of living cells acquired × number of beads acquired / 1.0 × 104. Data are representative of 3 independent experiments with similar results.