Expression of OPB in L. major
Western immunoblot showing the expression of OPB in the three life cycle stages of L. major.
Lane 1 = procyclic promastigotes, 2 = metacyclic promastigotes, 3 = amastigotes. Purified anti-OPB antibody was used, with anti-T. brucei elongation factor 1α (EF-1α) antibody as a loading control. A lysate of 5 × 106 cells was run per lane.