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. Author manuscript; available in PMC: 2011 Jul 7.
Published in final edited form as: Mol Biochem Parasitol. 2010 Sep 29;175(1):49–57. doi: 10.1016/j.molbiopara.2010.09.003

Fig. 4.

Fig. 4

Reduction of serine peptidase activity in Δopb

A: Effect of deletion and re-expression of OPB in L. major promastigotes on the cleavage of 5 μM Bz-R-AMC. Relative activity was calculated compared to WT for each experiment. For each assay, 6 × 106 cells were used, with the mean of between 5 and 11 experiments shown ± SEM.

B: Effect of over-expression of OPB in L. major promastigotes on the cleavage of 5 μM Bz-R-AMC. Relative activity was calculated compared to WT for each experiment. For each assay, 6 × 106 cells were used, with the mean of between 4 and 11 experiments shown ± SEM.

C: Fractionation of OPB. Western immunoblot showing partition of OPB to either soluble or membrane fraction of L. major. Lane 1; soluble fraction, 2; pelleted fraction. Purified anti-OPB antibody was used, with controls of anti-EF-1α and anti-GP63 antibodies.