Fig. 5.

Sequence alignment of L. major OPB and OPB2.

Alignment based on a multiple sequence alignment using eight OPB homologues performed by CLUSTALW. The catalytic domain of OPB is underlined in red and the propeller domain left blank. Predicted inserts of OPB2 into the OPB sequence are shown in pink. Identical residues are shaded, with those involved in the catalytic triad in red. Red, green and blue stars highlight all of the residues involved in the S1-S3 binding pockets, respectively. Solid stars represent hydrogen-bonding interactions while the hollow stars denote pi-cation or hydrophobic interactions. This shows that all of the important residues involved in the binding of antipain in OPB are conserved in OPB2.