Fig. 2.

Properties of membrane currents elicited by TMEM16A isoform expression. A, B. Representative whole-cell membrane currents from patch-clamp experiments on FRT cells with stable expression of TMEM16A(0) and TMEM16A(abc). Each panel shows superimposed currents elicited at membrane potentials in the range − 100 to + 100 mV with the indicated free Ca²⁺ concentration in the pipette solution. C, D. Corresponding current–voltage relationships from similar experiments (n = 7–48). The amplitude of the currents was taken as the maximal level reached at the end of the test pulse. E. Plot of the maximal conductance elicited at + 100 mV vs. the cytosolic free Ca²⁺ concentration for the (0) and the (abc) variants. Data are fitted as described in Materials and methods.