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. 2011 Jul;163(5):948–963. doi: 10.1111/j.1476-5381.2011.01305.x

Figure 8.

Figure 8

(A) Activation of 5-HT transporter (SERT) and the 5-HT2A but not the 5-HT1B receptor mediates 5-HT-activated phospho-Raf-1 (p-Raf-1), phospho-Raf-1 kinase inhibitor protein (p-RKIP) and phospho-atypical protein kinase Cζ (p-PKCζ) in normoxic CCL39 cells stimulated by 5-HT (1 µM; 5 min) but 5-HT activates phospho-ERK1/2 MAPK (p-ERK1/2) via SERT and 5-HT1B receptor. Antagonists added 45 min prior to the addition of 5-HT. 5-HT-induced phosphorylation of the respective signalling elements is shown in duplicate lanes. (B–E) Quantification of data is also shown representing four independent experiments with comparable outcomes [samples were adjusted for loading errors, using the tubulin blot density to standardize, prior to normalization relative to control (unstimulated) values]. Quantitative data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, significantly greater than control (unstimulated) value. †P < 0.05, ††P < 0.01, significantly less than 5-HT-treated cells.