Figure 3.
Impairment of astrocytic activity reduced the modulatory effects of proteinase-activated receptor-2 (PAR2) activation. (A) Pre-incubation of cultures with fluoroacetate (FAc; 10 µM, 3 h) abolished the SLIGRL (100 µM)-induced depolarization compared with control. (B) FAc pre-incubation reduced the decrease in action potential (AP) frequency observed following PAR2 activation compared to control. (C) Summary of the effects of FAc on neuronal membrane potential and AP frequency. (D) FAc pre-incubation significantly reduced the SLIGRL-induced increase in [Ca2+]i in astrocytes compared with control. *P < 0.05; **P < 0.01.