Table 1.

PCR fragments, primers, and conditions for all NCAM1 fragments analyzed

Fragment name	Forward primer (5′-3′)	Reverse primer (5′-3′)	HPLC temp (°C)
Exon 1	ggctgggactgtcactcatt	gcaaaccagattgagaattaaaa	60
Exon 2	gggtttcattcttgaacattgg	cctgagggctcctgctctac	60
Exon 3	ggggacttattagtcttttcgactt	gcagaagaagaaggaggctct	61
Exon 4	gaagcagctgttttccctca	tgaaaaagctagggaacttgg	60.5
Exon 5	tgcagatgctctctgactga	ccaaggttgtagcaatgcag	60
Exon 6	tgtctcttctccaggccatt	gactttgtgatgccccattc	59.5
Exon 7	tggtcgaaatcatgctactttg	attgtggcagagcagtgacc	60.5
VASE	ctaagggggaaaaaaagctggaca	tcatccactcccaacacagc	56
Exon 8	tgcatgccatcatttaaacc	attccaaggccctgaaactc	56, 60.5
Exon 9	ccttgggctctgacatgc	catcctgaccctgccttg	63.5
Exon 10	aatcatggcagtcatcctga	ttggagcccacctagagtca	61
Exon 11	tgaccatcccataggacactt	attgggctggcagggttag	60
Exon 12	atggtcttgggccaaactg	caggtggggacatctgagta	61
SEC	gagggtgatgccgagaaggaa	cacacggagggaacaccaaga	57
Exon 13	gaaatagaattgctggaccaaa	aaggtgggctgggaaaag	57
Exon 14	cctgtcactccatcccattc	cagggttctggtgaagtctga	60
Exon 15	tcccgtaagttttgcctattg	caagcaagttgtcagggttg	60.5
Exon 16	gtctggaggtctcgcatctc	caaacctcagcaaggtggac	63
Exon 17	gccttgggttgagtcatagc	gggtctctacggagcaggt	63
Exon 18	agaccgtggtctcagtggtt	tggaaatgctctggtgaagc	60.5
Promoter 1	gagggtttcagtgttctaggc	aagaaaactccgatgtttggaa	54
Promoter mid	ttttcttcgggttatttctgga	ccagccttccttaatcagca	56.5
Promoter 2	ctgattaaggaaggctgggta	tttttgcagaattgtttcctg	63