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. Author manuscript; available in PMC: 2012 Jun 26.
Published in final edited form as: Mol Cell. 2011 Jun 24;42(6):817–825. doi: 10.1016/j.molcel.2011.06.005

Figure 4. NanoRNA-mediated priming of transcription initiation in vivo leads to changes in gene expression.

Figure 4

A. and B. Effect of Orn depletion on gene expression in P. aeruginosa as determined by DNA microarray. Data were derived from the analysis of transcripts isolated from cells of strain JS1.F harboring plasmid pPSV35 (non-depleted), and cells of strain JS3.F harboring plasmids pPSV35 (Orn-depleted), pPAOrn (Orn-depleted + Orn), or pNrnB (Orn-depleted + NrnB). The table in panel A shows the number of genes whose expression changes by a factor of 2 or more upon depletion of Orn-VDAS4, or upon depletion of Orn-VDAS4 in the presence of either wild-type Orn (supplied by plasmid pPAOrn), or NrnB (supplied by plasmid pNrnB). Panel B shows a heatmap representation of the 1,158 genes whose expression changes by a factor of 2 or more upon depletion of Orn-VDAS4. Also shown are the corresponding effects on expression of these genes when either wild-type Orn, or NrnB, are supplied ectopically and Orn-VDAS4 is depleted.