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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cell Death Differ. 2010 Mar 19;17(9):1381–1391. doi: 10.1038/cdd.2010.25

Figure 3.

Figure 3

Dexamethasone downregulates Lck to inhibit TCR signaling. (a) Single cell calcium measurements in unstimulated WEHI7.2 cells treated with 10−8 M dexamethasone for 20 h. Representative traces are shown. (b) Cells were treated as in a, but stimulated with anti-CD3 antibody (2 μg/ml) 1 min into the calcium trace. (c) Quantification of oscillating cells (≥ three spikes) in a and b. (d) Cells were transfected with control (nontargeting) siRNAs or those that specifically target Lck and incubated for 24 h. Lysates were subjected to western blotting for Lck. (e) Representative calcium traces in nontargeting or Lck siRNA-transfected cells after stimulation with anti-CD3 (2 μg/ml). (f) Quantification of oscillating cells in e. Data are representative of multiple independent experiments. Error bars represent the S.E.M.