Figure 1.

The electroporation procedure: A. Electroporation occurs when an applied external field exceeds the capacity of the cell membrane. The formation of permeable areas happens in the frame of less than a second whereas resealing happens over minutes. As the resting transmembrane potential is negative on the inside respective to the outside, the first part of the membrane that will be permeabilised is the pole facing the positive electrode. The positive electrode should be imagined in the left of the picture and the negative electrode on the right. Pulses were delivered to a cell suspended in medium containing propidium iodide and after the pulses propidium iodide is trapped within the cells [9]. B. The application of pulses to skin tumours must be preceded by local or general anaesthesia, in local anaesthesia the lidocain is injected around the metastasis. C. The cliniporator equipment allows monitoring of voltage and current during the pulse. D. A treatment situation is shown where a patient is receiving local injection of bleomycin followed by application of pulses under local anaesthesia. The application of pulses lasts only a few minutes in total.