Figure 2.

TRAP assay of inhibition of telomerase activity upon addition of 20 base 2′-MOE RNA/DNA chimeras XI and XII to cell extract at 37°C. (A) Triplicate assay of match oligomer XI. (B) Triplicate assay of mismatch-containing oligomer XII. The internal amplification standard (ITAS) is noted. At the highest concentration ITAS DISAPPEARS because of inhibition of Taq polymerase by PS-substituted oligomers.