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Expression of wnt3, β-catenin, and ZEB1 in HK-2 cells. (A) Western blot analysis of wnt3, β-catenin, and ZEB1. Lanes 1—3 are 10% FBS group, 10% sham operation serum group, and 10% 5/6 nephrectomized rat serum group, respectively. (B) wnt3, β-catenin, and ZEB1 protein levels. Data were expressed versus β-actin and compared with ANOVA. Notes: The experiment was repeated three times with similar results.
* Denotes p < 0.05 versus 10% sham operation serum group.
