Skip to main content
. 2011 May 6;20(15):3042–3051. doi: 10.1093/hmg/ddr208

Figure 2.

Figure 2.

Lack of interaction between R215H mutant and NRXN-expressing HEK 293T cells. (A) Cells expressing the wild-type NLGN2 (green) were mixed with cells expressing NRXN1β (Red), and merged image showed the aggregation of NLGN2-expressing and NRXN1β-expressing HEK 293T cells. (B) GFP-expressing and NRXN-expressing HEK 293T cells did not aggregate. (C–F) The aggregation ability of each mutant was assessed and representative merged images were showed for each mutant, including R215H (C), V510M (D), R621H (E) and A637T (F). (G) Quantification of the aggregation assay shows that R215H mutation had significantly impaired aggregation compared with the wild-type, while V510M, R621H and A637T mutants did not show significant differences. *P< 0.05, **P< 0.01. The scale bar is 60 μm.