Fig. 11.

Topology of the Trypanosoma cruzi kDNA minicircle integrated into the genome of a Chagas' case and knockout of the CLEC5A gene. The scheme shows kDNA integration into the chromosome 7 CLEC5A gene open reading frame in continuity with LINE-1 at RP11-707F14. The tpTAIL-PCR amplicon that was cloned and sequenced shows that the s67 reverse kDNA primer anneals at the 5′ end and that the L1-5 primer anneals at the 3′ end. The kDNA (light blue, variable region) extends from nucleotides 1 to 286, and the host DNA (green) extends from nucleotides 270 to 1230. E values for kDNA and for host DNA are highly statistically significant. (Scheme reproduced from reference 174a with permission of the publisher.)