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. 2011 Jul;24(3):592–630. doi: 10.1128/CMR.00063-10

Fig. 12.

Fig. 12.

Trypanosoma cruzi infection is established in a Gallus gallus embryo. (A) Positive-control T. cruzi trypomastigote fluorescein labeled with antibody from a Chagas' disease patient. The inset shows a fluorescein-labeled amastigote stained with Chagas' disease patient antibody diluted 1:128 in PBS (pH 7.4). (B) Section (HE stained) (magnification, ×100) from an uninfected chicken embryo which remained unstained with the anti-T. cruzi antibody diluted 1:128. (C) Background staining of the same control section treated with fluorescein-labeled anti-T. cruzi antibody. (D) Infected chicken embryo endoderm and mesoderm parasitized cells stained green by the fluorescein-labeled anti-T. cruzi antibody diluted 1:128. (E) X-gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) blue-stained β-galactosidase-expressing T. cruzi Tulahuen-infected chicken embryo endoderm and mesoderm cells. Control serum from an uninfected donor lacking anti-T. cruzi antibody did not stain parasitized embryo cells. (Reprinted from PLoS Neglected Tropical Diseases [402].)