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. 2001 Apr 15;29(8):1801–1807. doi: 10.1093/nar/29.8.1801

Figure 3.

Figure 3

Detection of transcription of both pUSAG15 and pUSAG16 in HeLa nuclear extract. The in vitro transcription reactions with pUSAG15 and pUSAG16 were performed in HeLa nuclear extracts. (A) Detection of RNA transcripts synthesized from pUSAG15 and pUSAG16 plasmid DNA in HeLa nuclear extract. Lane 1, pUSAG15; lane 2, pUSAG16. (B) Reverse transcription assay to determine the initiation sites of RNA transcripts synthesized from pUSAG15 and pUSAG16 plasmid DNA in HeLa nuclear extract. Lane 1, no DNA control; lane 2, pUSAG15; lane 3, pUSAG16 (the arrows indicate specific RNA transcripts initiated from the SV40 promoter).